What is ELISA and how does it work

 

A common method in biochemistry and immunology for identifying and measuring proteins, peptides, antibodies, and hormones is the enzyme-linked immunosorbent assay (ELISA). The fundamental idea behind ELISA is to immobilize a particular antigen or antibody onto a solid surface, such a microplate, and then monitor its binding to a corresponding antibody or antigen that has been enzyme-labeled. An ELISA kit distributor is a company that specializes in the distribution of enzyme-linked immunosorbent assay kits for research, diagnostics, and drug discovery.

The enzyme catalysis a colorimetric or fluorescent reaction that may be seen using a spectrophotometer or fluorimeter when a substrate is introduced. This makes it possible to precisely quantify the target molecule in a sample. The excellent specificity, sensitivity, and repeatability of ELISA make it a popular tool in research, clinical diagnostics, and drug development.

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